High Sens. RNA ScreenTape Sample Buffer from Agilent Technologies

Product Specs
ItemHigh Sens. RNA ScreenTape Sample Buffer
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog Number5067-5580
Sensitivity100 pg/µL
Sample Volume2 µL
Concentration1 mM EDTA, 10 mM Tris

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

Company Profile

Website: www.agilent.com

(1) Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculatureBrain, Behavior, & Immunity - HealthDecember 1, 2023Marina Wakid, Daniel Almeida, Zahia Aouabed, Reza Rahimian, Maria Antonietta Davoli, Volodymyr Yerko, Elena Leonova-Erko, Vincent Richard, René Zahedi, Christoph Borchers, et al.cat. no. 5067–5577) or TapeStation High Sensitivity RNA ScreenTape sample buffer (Agilent, cat. no. 5067–5580)•SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio Inc., cat. no. 634485)•NucleoMag

(2) Transcriptomes of aging brain, heart, muscle, and spleen from female and male African turquoise killifishScientific DataOctober 12, 2023Alan Xu, Bryan B. Teefy, Ryan J. Lu, Séverine Nozownik, Alexandra M. Tyers, Dario R. Valenzano, Bérénice A. Benayouninstructions. RNA quality was assessed using high sensitivity RNA screen tapes (Agilent cat# 5067–5579, 5067–5580) on Agilent Tapestation 4200 to obtain the RNA Integrity Number (RIN). Samples with a RIN score of <4

(3) Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sitesHeliyonSeptember 9, 2023Laura Atkinson, Jack CD. Lee, Alexander Lennon, Divya Shah, Nathaniel Storey, Sofia Morfopoulou, Kathryn A. Harris, Judy Breuer, Julianne R. BrownRNA ladder,Agilent Technologies,5067–5581 High sensitivity RNA sample buffer,Agilent Technologies,5067–5580 High sensitivity RNA screen tape,Agilent Technologies,5067–5579 KAPA RNA HyperPrep Kit,Roche,8098093702 KAPA RNA HyperPrep Kit

(4) Calling Cards: a customizable platform to longitudinally record protein-DNA interactions over time in cells and tissuesbioRxivJune 15, 2023Allen Yen, Chase Mateusiak, Simona Sarafinovska, Mariam A. Gachechiladze, Juanru Guo, Xuhua Chen, Arnav Moudgil, Alexander J. Cammack, Jessica Hoisington-Lopez, MariaLynn Crosby, et al.C2432) RNA kit TURBO DNase High Sensitivity RNA ScreenTape (Agilent 5067-5579) and Sample Buffer (Agilent 5067-5580) Qubit RNA Assay Kits (ThermoFisher Q32852, Q10211, or Q33224) Refrigerated centrifuge (Eppendorf 5430R) Rotor

(5) Moderate intrinsic phenotypic alterations in C9orf72 ALS/FTD iPSC-microglia despite the presence of C9orf72 pathological featuresFrontiers in Cellular NeuroscienceJune 6, 2023Ileana Lorenzini, Eric Alsop, Jennifer Levy, Lauren M. Gittings, Deepti Lall, Benjamin E. Rabichow, Stephen Moore, Ryan Pevey, Lynette M. Bustos, Camelia Burciu, et al.and quality with Agilent High Sensitivity RNA ScreenTape and buffer (Agilent, Cat. No. 5067-5579 & 5067-5580). For each RNA sample, an indexed, Illumina-compatible, double-stranded cDNA whole transcriptome library

(6) The scaffolding function of LSD1/KDM1A reinforces a negative feedback loop to repress stem cell gene expression during primitive hematopoiesisiScienceJanuary 20, 2023Mattie J. Casey, Alexandra M. Call, Annika V. Thorpe, Cicely A. Jette, Michael E. Engel, Rodney A. Stewartwas not utilized. RNA quality was assessed using the Agilent ScreenTape Assay (Agilent 5067-5579 and 5067-5580) and the Illumina TruSeq Stranded Total RNA kit with Ribo-Zero Gold (Illumina 20020598) was used for

(7) PepSeq: a fully in vitro platform for highly multiplexed serology using customizable DNA-barcoded peptide librariesNature ProtocolsNovember 16, 2022Sierra N. Henson, Evan A. Elko, Piotr M. Swiderski, Yong Liang, Anna L. Engelbrektson, Alejandra Piña, Annalee S. Boyle, Zane Fink, Salvatore J. Facista, Vidal Martinez, et al.cat. no. 5067-5579) High Sensitivity RNA ScreenTape sample buffer (store at 4 °C; Agilent, cat. no. 5067-5580) RNA ScreenTape (store at 4 °C; Agilent, cat. no. 5067-5576) RNA ScreenTape sample buffer (store

(8) Dual genome-wide coding and lncRNA screens in neural induction of induced pluripotent stem cellsCell GenomicsNovember 9, 2022David Wu, Aunoy Poddar, Elpiniki Ninou, Elizabeth Hwang, Mitchel A. Cole, S. John Liu, Max A. Horlbeck, Jin Chen, Joseph M. Replogle, Giovanni A. Carosso, et al.Barcoding,10x Genomics,"Cat# PN-1000075, PN-1000153, PN-1000079" High Sensitivity RNA ScreenTape,Agilent,Cat# 5067-5580 High Sensitivity D1000 DNA ScreenTape,Agilent,Cat# 5067-5584 High Sensitivity D5000 DNA ScreenTape,Agilent,Cat#

(9) Hand Dissection of Caenorhabditis elegans IntestinesJournal of Visualized ExperimentsOctober 3, 2022Jessica L. Hill, Andrew Moore, Robert T. P. Williams, Erin Osborne Osborne Nishimuraassesment of total RNA quality and quantity High Sensitivity RNA ScreenTape Sample Buffer,Agilent,5067–5580,for assesment of total RNA quality and quantity HostZERO Microbial DNA Kit,Zymo Research,D4310,Isolation

(10) Distinct gene expression dynamics in developing and regenerating crustacean limbsProceedings of the National Academy of SciencesJuly 5, 2022Chiara Sinigaglia, Alba Almazán, Marie Lebel, Marie Sémon, Benjamin Gillet, Sandrine Hughes, Eric Edsinger, Michalis Averof, Mathilde Parisquality was assessed with TapeStation 2200 (Agilent RNA ScreenTape High Sensitivity System: #5067-5579, #5067-5580, and #5067-5581), and 1 ng of RNA was used for cDNA synthesis (SMART-Seq v4 Ultra Low Input RNA Kit;

(11) Nano pom-poms prepared exosomes enable highly specific cancer biomarker detectionCommunications biologyJuly 4, 2022He N, Thippabhotla S, Zhong C, Greenberg Z, Xu L, Pessetto Z, Godwin AK, Zeng Y, He M.quantified by High Sensitivity RNA ScreenTape Assay using Agilent TapeStation 2200 (Agilent, USA, 5067-5579, 5067-5580). Total RNA was stored at −80 °C until small RNA Library preparation. The QIAseq miRNA Library is prepared

(12) Bacterial growth in multicellular aggregates leads to the emergence of complex life cyclesCurrent BiologyJuly 1, 2022Julia A. Schwartzman, Ali Ebrahimi, Grayson Chadwick, Yuya Sato, Benjamin R.K. Roller, Victoria J. Orphan, Otto X. CorderoSanta Clara, CA, USA",Cat#5067- 5579 HS RNA screen tape buffer,"Agilent, Santa Clara, CA, USA",Cat#5067- 5580 HS RNA screen tape ladder,"Agilent, Santa Clara, CA, USA",Cat#5067- 5581 Ribominus Yeast and Bacteria

(13) Impact of secondary TCR engagement on the heterogeneity of pathogen-specific CD8+ T cell response during acute and chronic toxoplasmosisPLoS pathogensJune 1, 2022Shallberg LA, Phan AT, Christian DA, Perry JA, Haskins BE, Beiting DP, Harris TH, Koshy AA, Hunter CA.ScreenTape,Agilent,5067–5579 High Sensitivity RNA Ladder,Agilent,5067–5581 High Sensitivity RNA Buffer,Agilent,5067–5580 AMPure XP beads,Beckman Coulter,A63881 Foxp3 / Transcription Factor Staining Buffer Set,eBioscience,00-5523-00 Reagents.

(14) Zebrafish models of alx-linked frontonasal dysplasia reveal a role for Alx1 and Alx3 in the anterior segment and vasculature of the developing eyeBiology OpenMay 15, 2022Baul Yoon et al.Kit (Qiagen 74134) and RNA quality was assessed by Agilent High Sensitivity RNA ScreenTape (Agilent, 5067-5580). cDNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library kit (NEB, E7765S)

(15) Secretory defects in pediatric osteosarcoma result from downregulation of selective COPII coatomer proteinsiScienceApril 15, 2022Rachael K. Wood, Ashley R. Flory, Melissa J. Mann, Lindsay J. Talbot, Linda M. HendershotRiboGreen assay,Life Technologies,R32700 High Sensitivity RNA ScreenTape assay,Agilent,5067-5579; 5067-5580 LabChip RNA Pico Sensitivity assay,Perkin Elmer,CLS960012 TruSeq Stranded Total RNA Library Prep Kit,Illumina,20020599 Caliper

(16) Synthetic DNA spike-ins (SDSIs) enable sample tracking and detection of inter-sample contamination in SARS-CoV-2 sequencing workflowsNature MicrobiologyJanuary 1, 2022Kim A. Lagerborg et al.RNA using a T7 transcription kit (NEB E2050), quantified by RNA screen tape (Agilent 5067–5579 and 5067–5580), then diluted in water to 10fM (6,000 copies/μL), 1fM (600 copies/μL), 100aM (60 copies/μL), and 10aM

(17) The Extracellular Milieu of Toxoplasma 's Lytic Cycle Drives Lab Adaptation, Primarily by Transcriptional ReprogrammingmSystemsDecember 21, 2021Vincent A. Primo, Yasaman Rezvani, Andrew Farrell, Connor Q. Murphy, Jingjing Lou, Amir Vajdi, Gabor T. Marth, Kourosh Zarringhalam, Marc-Jan Gubbelsmeasuring the RNA integrity number (RIN) using Agilent’s TapeStation (kit catalog no. 5067-5579 and 5067-5580). Illumina’s library prep kit (RS-122-2102) was used to generate ∼281-bp cDNA fragments, which were

(18) Genome annotation with long RNA reads reveals new patterns of gene expression and improves single-cell analyses in an ant brainBMC BiologyDecember 1, 2021Emily J. Shields, Masato Sorida, Lihong Sheng, Bogdan Sieriebriennikov, Long Ding, Roberto BonasioScientific) and RNA integrity was checked using High Sensitivity RNA ScreenTape (Agilent #5067-5579, 5067-5580, 5067-5581) on a TapeStation 2200 or 4200 (Agilent). 250–500 ng extracted RNA was used to prepare libraries

(19) Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seqNature ProtocolsDecember 1, 2021Nikolay L. Malinin et al.Sensitivity Screen Tape (Agilent cat. no 5067-5579) RNA High Sensitivity Sample Buffer (Agilent cat. no. 5067-5580) Mammalian Cell culture and nucleofection 24 Well Cell Culture Plate, Flat Bottom with Lid, Tissue Culture

(20) Wisdom of the crowds: A suggested polygenic plan for small-RNA-mediated regulation in bacteriaiScienceOctober 22, 2021Omer Goldberger, Jonathan Livny, Roby Bhattacharyya, Orna Amster-ChoderKit,Illumina,Cat#MRZB12424 SMARTScribe Reverse Transcriptase,Takara Bio,Cat#639538 High Sensitivity RNA Screentape reagent,Agilent,Cat#5067-5580 RNA Clean & Concentrator Kit,Zymo Research,Cat#R1013 AccuPrime Taq HiFi Kit,Life Technologies,Cat#12346094 High

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